Research Facilities



UV-Vis. Spectrophotometer

Model: Perkin Elmer Lambda 35
Type: Scanning double-beam spectrometer for the UV/Vis range
Two Radiation Sources: Deuterium & Tungsten Filament Lamp
Wavelength Scan Range: 190 - 1100nm
Grating (Monochromator): Holographic concave grating with 1053 lines/mm.
Detector: Photodiodes

Inverted Fluorescence Microscope

Model: Nikon Eclipse TE300
Magnifications: 4X, 10X, 20X, 40X, 60X
Lamp: Mercury and Halogen
Filters: ND2, ND16, NCB11, GIF
Power Supply: TE-PS30 in combination with the 6V-30W Illumination Pillar.

Raman system

Overview:
The Horiba Jobin Yvon LabRAM HR micro Raman system provides ultra high spectroscopic resolution and a unique wavelength range capability that provides both great flexibility and high performance to acquire Raman Spectra with high resolution. It is an integrated, simple to use, and high stability bench-top instrument. This is used to study the raman scattering properties of cancerous and normal tissues for both invivo and invitro conditions.  In addition to this biofluids are also studied.
- Equipped with two gratings this system offers high and medium resolution of the spectrum.
-Multiple lasers (Argon-Ion Laser at 488nm, Helium Neon Laser at 633nm and 785nm diode laser)
-Large 1024 pixel CCD chip dimensions
-Fast Map imaging modes - DuoScan
Technical Specifications:
-Confocal microscope
-600 gr/mm and 1800 gr/mm gratings
-3 objectives, 10x, 50x, 100x, 60x water immersion and 50x long working distance
-Automated XYZ scanning stage.
-Full area CCD detector, 1024 x 256 pixel, -70 C, air cooled
-Labspec 5 software
- Spectral ID and Chemometric software
System Usages:
This system with its unique features is used for raman characterisation of biological and crystalline samples both in solid and liquid phases with high spectral resolution.

Fluorolog – 3

Overview:
The FluoroLog®-3 is a unique, modular system which allows the researcher to interchange a versatile range of accessories to correspond perfectly with the characteristics of a given sample. This instrument can perform both steady-state and TCSPC lifetime fluorescence spectroscopy with the highest sensitivity and fast time resolution.
Steady State:
Source : 450-W Xenon Lamp
Double grating excitation and emission monochromator
Detector: PMT (R928P) Hamamatsu Photonics, Japan
Time Correlated Single Photon Counting (TCSPC)
Lifetime fluorescence spectroscopy investigates the change in fluorescence over time of a sample when irradiated with UV, visible, or near-IR light. It aids in understanding the micro environment of the fluorophores.
Excitation source: 265, 280, 310 and 460 Nano LED

Fluoromax 2 for steady state fluorescence measurements

Fluoromax-2, ISA; Jobin Yvon-Spex, Edison, NJ.
The excitation source :150 W ozone free xenon arc lamp
Single monochromator
Detector: Photomultiplier tube (R928P; Hamamatzu, Shizuoka-Ken, Japan).

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