Center for Biotechnology was established in 1987 in Anna University
with a financial support from Department of Biotechnology, Delhi,
University Grants Commission, Delhi and Anna University with
to provide educational and training facilities in different
areas of Biotechnology
to carry out fundamental research in the frontier areas of Biotechnology
to promote research and consultancy activities in the development
of various areas of Biotechnology
centre has a well defined independent building of about 1000
sq. meters at Taramani Campus with facilities for carrying out
research in various areas of biotechnology, housing modern infrastructure
to conduct work in Bio process Technology, Molecular Biology,
Cell Biology, Immunology, etc. The Administrative building is
located in the Main Campus, besides to it a separate Academic
Complex is situated exclusively to offer teaching programme
for the B.Tech. Industrial Biotechnology and M.Tech Biotechnology.
Computerised Departmental Library of the Center for Biotechnology
subscribes nearly 40 journals specialized in the filed of Biotechnology
at National and International level. It has a collection of
more than 2000 volumes on various topics. E-mail, CD-ROM and
Audio-Visual facilities are also available.
Biotechnology Information Sources(BTIS)
centre is one of the sub-distributed information centres(Sub-DIC)
set up by the Department of Biotechnology, Government of India.
The Sub-DIC has 3 Pentium Servers with 486 nodes. This is meant
for providing information service to users in the city as well
as to train students in Bioinformatics. The centre has software
on Bioprocess modeling, Symbolic Manipulation, Molecular modeling,
MATLAB. It also provides Internet facility to students. It is
connected via fibre optic cable to the Campus network.
Technology Business Incubator(TBI)
for Biotechnology, Anna University has started Technology Business
Incubator with the support of Department of Science and Technology,
India and UNAPCTT. TBI will provide infrastructure on demand
to the industries/entrepreneurs to evaluate the techno-economic
feasibility of the project. TBI Part is located at Taramani
Campus with an objective:
encourage entrepreneurs to develop their technological initiatives
to commercial viability
To provide sufficient infrastructure for these entrepreneurs
so that minimal loss of time can be ensured, before the
provide linkages with the technical expertise available
in Anna University and the experimental facilities available
at various laboratories elsewhere
well - established facility has been developed at Taramani;
companies can utilize this facility to develop products
of interest. Specific MOU's will be signed between the Company
and University, detailing out the cost and the time frame
of operations. The TBI is well is well equipped with high-end
infrastructure, lab space. The support of technical knowledge
from CBT is also available.
Foundation of Advancement of Pharmaceutics(FAP)
foundation (FAP) is a Chennai based Scientific Research Organization
signed a Memorandum of Understanding with Anna University on
July 3, 2002. Strategic intent behind the Industry and Academia
is to integrate resources and to do much more than has been
was set up with an objective of investigating molecular entities
and pharmaceutical substances, with particular emphasis on those
having ethanobotanical provenance. Biotechnologically derived
therapeutics, vaccines, and diagnostics also come within its
by National and International Agencies
DR ARUN BALAKRISHNAN, PROFESSOR & DIRECTOR
Culture and Drug Discovery:
focus of current research has been in the direction of understanding
the mechanisms of pathogenesis in two major tropical infections.
In addition, mm interest has also been in the interplay between
traditional medicine and the modern tools of biotechnology,
aimed towards the development of new drugs for the treatment
of diabetes and cancer.
filarial proteins elicit strong immunological reactions in humans
leading to the chronic manifestations in human lymphatic filariasis
such as lymphatic occulusion, fibrosis, oedema and in some cases
tropical pulmonary eosinophilia(TPE). Our focus has been to
investigate the molecular switches that control the regulation
of cell growth and apoptpsis during a pathogenic state. We have
investigated various key molecules in this pathogenic cascade
Pathogenesis of EPEC diarrhoea:
is a heterogeneous group of organisms, traditionally considered
to comprise 12 O serogroups that cause symptoms ranging from
acute self-limiting gastroenteritis to persistent life threatening
diarrhoea, especially among the very yound. Extensive studies
over several years on the interaction of EPEC with cultured
cells have led to the generation of a three-stage model of pathogenesis.
Bioscreening and isolation of medicinal properties from plant
sources towards new drug development: Medicinal plants
have been used for the treatment of different diseases in traditional
medicines for several generations. Our goal has been to establish
interplay between traditional medicine practiced. in South India
and modern tools of biotechnology, to determine the specific
bioactivities of the medicinal plants using invitro techniques.
The DBT, Government of India has funded several projects in
DR. P. KALIRAJ, PROFESSOR & DIRECTOR
WHO-sponsored Filarial Genome project:
group was involved in sequencing a total of 17, 000 ESTs comprising
of 7000 genes of B.malayi. Functional genomic analysis revealed
that 30% of the genes were unique for filarial parasite, 27%
being structural genes and 20% being enzymes.
Immuno diagnosis of human lymphatic filariasis:
Kaliraj's group has identified WbSXP-1 as a promising and a
more reliable candidate for the identification of active infections
in both bancroftian and brugian filariasis. Simple and rapid
qualitative Immunodiagnostic test kits for the identification
of antigen(monoclonal antibody to WbSXP-1 based) and antibody
(recombinant filarial antigen WbSXP-1 based) from individuals
with W.banicrofti / B.malayi / mixed infections.
DR. R.B NARAYANAN, ASSISTANT PROFESSOR
Immunopathology of Filariasis:
T cell and macrophage function in filarial patients: T
cells and macrophages are purified from the blood of patients
with bancroftian filariasis. Purified filarial recombinant proteins
and crude parasite antigens are allowed to interact with these
cells and functional status like lymphoproliferation, cytosine
production and other biological mediators release is assessed.
Wolbachia genes: Wolbachia is an endosymbiont
bacteria that resides in the live filarial worms of either sex
or microfilaria. We have identified new genes from wolbachia
of W.bancrofti and extended these studies to the cattle filarial
parasite Setaria digitata. These genes are being cloned, expressed,
purified and used for immunological studies in filarial patients.
Identification of new genes from W.bancrofit: Lymphodema
is a severe clinical condition in filarial patients with chronic
pathology. At the present time there is no reliable test available
to distinguish between the lymphodema of filarial or non-filarial
Production of recombinant proteins to White Spot Syndrome Virus
Spot Syndrome Virus(WSSV) infects a sizable population of shrimps
and crabs. Our lab has recently developed an immunodiagnostic
assay to detect WSSV in shrimps and its possible commercialization
is being explored.
Spermicidal effects of neem oil and its fractions:
oil fractions have various biological functions and therapeutic
uses. This is a collaborative project between the Centre for
Biotechnology and TTK LIG limited. Chennai to screen neem oil
fractions for spermicidal action on human sperms.
DR.P.GAUTAM, ASSITANT PROFESSOR
Lipase Secretion & Optimization:
have been using the Candida rugosa (culture from DSM, Germany),
for optimizing production of lipases. We have shown that vegetable
oils given as a carbon source increase the yield of lipases.
We have developed both quantitative and qualitative assays for
lipases. We are currently studying a related organism Schizophyllum
commune for production of lipases and oxidizing enzymes for
Bioleaching and Bioremediation:
have developed new analytical methods for following the oxidation
of Fe3+by Thiobacillus ferrooxidans. A new solid
matrix for growth of Thiobacillus ferrooxidans has been designed
in the laboratory. The use of Thiobacillus ferrooxidans in the
removal of iron and inorganic sulphur from lignite for cleaner
burning is being investigated.
Computational Biology & Bioinformatics:
group has been carrying molecular dynamics simulation of lipases
and metal containing enzymes. We have built a 16 node cluster
for doing parallel computation. In collaboration with Prof.
Ramesh Anishetty, Institute of Mathematical Sciences, we have
developed a knoweledge-based method to understand protein folding.
DR. K. SANKARAN, ASSISTANT PROFESSOR
Bacterial Lipid Modification-novel protein engineering applications:
ELISA to Biosensors, many applications require hydrophilic proteins
to be attached to hydrophobic man-made surfaces like plastic
overcoming coatability and binding problems. Pioneering work
done by Dr. Sankaran in association with Prof. Henry C. Wu,
USA showed in E.coli and other bacteria that a three enzyme
pathway attaches a lipid adapter to the N-terminus of hydrophilic
proteins. This work was partly funded by AICTE, India.
Molecular pathogenesis and early diagnosis of Diarrhoeal pathogens:
50% of infantile mortality, malnutrition and poor physical and
low IQ are due to bloody and persistent diarrhoea caused by
Shigella and E.coli. Availability of simple, rapid and low-cost
but sensitive early diagnostic methods designed for peripheral
clinical labs operating with minimum facility will drastically
reduce this burden in bulk of the affected population living
in poor sanitary conditions.
DR. GEETHA MUTHUKUMARAN, VISITING FACULTY
In vitro Evolution of Lipase Genes:
vitro molecular evolution is a powerful technique of producing
proteins with vastly improved activity parameters in a non-targeted
manner. This method, also referred to as gene shuffling, has
been used to generate enzymes over a thousand-fold more active
than the original enzyme. Lipase is a hydrolytic enzyme that
is widely used in various industries.
Investigation of the Pharmacologic effects of Stevioside, a
is a perennial herb whose leaves are used as a natural sweetener
in several Asian countries. The sweetening effect is attributed
to two glycosidic compounds-stevioside and rebaudioside - which
are several times sweeter than sucrose.
DR.V. MURUGAN, ASSISTANT PROFESSOR
Recombinant protein production:
proteins, Prophylactic protenis, Diagnostic proteins, Industrial
enzymes and Food preservatives form an important group of high-value-low-volume
products in biotechnology industries. Isolation of these protenis
from the respective natural sources is often insufficient and
economically not viable.
group is actively involving in the strain improvement and the
biotransformation studies for the production of organic acids.
Conventional mutation studies involving physical and chemical
mutagens as well as molecular biological approaches are being
applied to increase the efficiency of biotransformation.
Generation of viral resistant transgenic shrimps:
spot disease is one of the important viral diseases. By considering
the detrimental effect of this disease in shrimp population
this virus has been characterized as a deadly one. Our research
focus on identification of novel genes involved in the pathogenesis
of white spot syndrome towards the development of transgenic
viral resistant shrimp.
DR. SANJOY GHOSH, ASSISTANT PROFESSOR
Large Scale Production of Recombinant SXP1 Protein:
production of recombinant proteins involves, several steps,
which include genetic manipulation of the host organism, optimization
of parameters for high yield, high level expression and down
stream processing of the desired product. The work is in progress
is mainly on the optimization of process parameters, designing
the media, design of feed flow patterns for various mode of
reactors, mathematical modeling and simulation of the recombinant
protein production based on mechanism of induction involved.
Extractive Fermentation of Lipase Using Aqueous Two-Phase System:
two-phase has already been established as an excellent tool
in the field of biomolecules separation and purification, especially
for proteins. We are trying to utilize this wonderful system
for purification and extractive fermentation of lipase enzyme
from Candida rugosa.
DR. S. MEENAKSHISUNDARAM, BUSINESS MANAGER (TBI)/ASSISTANT PROFESSOR
and Purification of r-DNA derived industrially important
proteins under different metabolic control system
Control and Modeling of biological systems & development
of Supervisory Control and Data Acquisition System
theoretical and control aspects of bioreactors operating
under batch or continuous conditions
productivity fermentations using Fed Batch cultivation and
Total cell retention culture
of online analytical system for monitoring the growth, substrate
utilization and product formation
of novell strains for the biodegradation and bioconversion